General description

Heat-killed, formalin-fixed Staphylococcus aureus cells (Cowan I strain) that bear a high cell-surface density of protein A and have been pickled by the method of Kessler. Useful as a solid-phase IgG-binding reagent due to the high affinity interaction of protein A with the Fc domain of IgG. PANSORBIN™ cells work best when the antibody is human (IgG1, IgG2, IgG4), rabbit IgG (all isotypes), or mouse (IgG2a, IgG2b, IgG3). Most common applications include immunoprecipitation and mitogenic stimulation of B lymphocytes.

Legal Information

PANSORBIN is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kierszenbaum, F., et al. 1991. Immunology74, 317.Meikle, P.J., et al. 1991. J. Biol. Chem.266, 22569.Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.Murakami, H., et al. 1988. Biochem. J.256, 917.Kessler, S.W. 1975. J. Immunol. 115, 1617.

Packaging

1 g in Glass bottle

10, 50 g in Glass bottle

5 g in Plastic ampoule

Physical form

Supplied as a 10% suspension of Staphylococcus aureus cells in PBS, 0.1% NaN₃, pH 7.2.

Warning

Toxicity: Standard Handling (A)